Journal
/
Biology
/
HO-Endonuclease Oluşum kantitasyonu ve Analiz Tavlama Tek-Strand kromozomal Translokasyonlar Uyarılmış Saccharomyces cerevisiae</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
Please note that all translations are automatically generated. Click here for the English version.

HO-Endonuclease Oluşum kantitasyonu ve Analiz Tavlama Tek-Strand kromozomal Translokasyonlar Uyarılmış Saccharomyces cerevisiae</em

Türkçe

Automatically Generated

14,426 Views

•

09:40 min

•

September 23, 2011

DOI:

10.3791/3150-v

DOI:
10.3791/3150-v

•

09:40 min
September 23, 2011

•

6 Views
, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Summary

Automatically generated

HO-uyarılmış translokasyon tahlil tek iplikli DNA, çift iplikli tatili diploid birden fazla lokusların kurulmasından sonra tavlama izler<em> Saccharomyces cerevisiae</em>. Bu mekanizma, iyonlaştırıcı radyasyon yüksek dozlarda maruz kalma yüksek ökaryotlar somatik hücre genomu düzenlenmeleri model olabilir.

Explore More Videos

QuantitationAnalysisFormationHO-EndonucleaseChromosomal TranslocationsSingle-Strand AnnealingSaccharomyces CerevisiaeGenetic VariationGenomic RearrangementsRepetitive ElementsEukaryotic GenomeRetrotransposon OriginLINEsSINEsExchange EventsGenome RearrangementsTranslocationsGene DosageGene ExpressionAutoimmune DiseasesCardiovascular DiseasesCancerHomologous RecombinationNon-homologous End Joining (NHEJ)Double-strand Breaks (DSBs)Mitotic CellsDNA ReplicationOxidative LesionsIonizing Radiation (IR)Exogenous DNA Damaging Agents

Read Article

Cite this Article

Liddell, L., Manthey, G., Pannunzio, N., Bailis, A. Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae. J. Vis. Exp. (55), e3150, doi:10.3791/3150 (2011).

Download .ris file

Copy

Share Video

.

Copy