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Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae</em
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Biology
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Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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Kwantificering en analyse van de vorming van HO-endonuclease chromosomale translocaties Gestimuleerd door Single-Strand Gloeien in Saccharomyces cerevisiae</em

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14,426 Views

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09:40 min

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September 23, 2011

DOI:

10.3791/3150-v

DOI:
10.3791/3150-v

•

09:40 min
September 23, 2011

•

6 Views
, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Summary

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De HO-gestimuleerde translocatie assay monitoren enkelstrengs gloeien na de oprichting van DNA dubbelstrengs breuken op meerdere loci in diploïde<em> Saccharomyces cerevisiae</em>. Dit mechanisme kan model genoom herschikkingen in somatische cellen van hogere eukaryoten na blootstelling aan hoge doses ioniserende straling.

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Liddell, L., Manthey, G., Pannunzio, N., Bailis, A. Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae. J. Vis. Exp. (55), e3150, doi:10.3791/3150 (2011).

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