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Quantificazione e analisi della formazione di HO-endonucleasi Stimolato traslocazioni cromosomiche da Single-Strand Ricottura in Saccharomyces cerevisiae</em
JoVE Journal
Biology
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JoVE Journal Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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Quantificazione e analisi della formazione di HO-endonucleasi Stimolato traslocazioni cromosomiche da Single-Strand Ricottura in Saccharomyces cerevisiae</em

italiano

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14,426 Views

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09:40 min

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September 23, 2011

DOI:

10.3791/3150-v

DOI:
10.3791/3150-v

•

09:40 min
September 23, 2011

•

6 Views
, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Summary

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L'HO-stimolato test traslocazione monitor singolo filamento di ricottura seguito alla creazione di DNA a doppio filamento a loci multipli in diploidi<em> Saccharomyces cerevisiae</em>. Questo meccanismo può modello di riarrangiamenti del genoma nelle cellule somatiche di eucarioti superiori in seguito all'esposizione ad alte dosi di radiazioni ionizzanti.

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Liddell, L., Manthey, G., Pannunzio, N., Bailis, A. Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae. J. Vis. Exp. (55), e3150, doi:10.3791/3150 (2011).

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