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La quantification et l'analyse de la formation de HO-endonucléase Stimulé par les translocations chromosomiques simple brin recuit dans Saccharomyces cerevisiae</em
JoVE Journal
Biology
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JoVE Journal Biology
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
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La quantification et l'analyse de la formation de HO-endonucléase Stimulé par les translocations chromosomiques simple brin recuit dans Saccharomyces cerevisiae</em

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14,426 Views

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09:40 min

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September 23, 2011

DOI:

10.3791/3150-v

DOI:
10.3791/3150-v

•

09:40 min
September 23, 2011

•

6 Views
, , ,
1Irell & Manella Graduate School of Biological Sciences, 2Department of Molecular and Cellular Biology,City of Hope Comprehensive Cancer Center and Beckman Research Institute, 3Department of Biochemistry and Molecular Biology,University of Southern California, Norris Comprehensive Cancer Center

Summary

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Le dosage de la translocation HO-stimulé surveille simple brin recuit après la création de l'ADN des cassures double brin à des loci multiples diploïde<em> Saccharomyces cerevisiae</em>. Ce mécanisme peut modèle de réarrangements génomiques dans les cellules somatiques des eucaryotes supérieurs après une exposition à des doses élevées de rayonnements ionisants.

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Liddell, L., Manthey, G., Pannunzio, N., Bailis, A. Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae. J. Vis. Exp. (55), e3150, doi:10.3791/3150 (2011).

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