This content is Free Access.
Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
Capítulos
- 00:05Título
- 01:16Determining HO-stimulated Translocation Frequencies
- 02:40Determining Plating Efficiencies
- 04:05Southern Blot Analysis
- 04:57Chromosome Blot Analysis
- 06:13Quantitation and Analysis of Chromosomal Translocations
- 09:21Conclusion
The HO-stimulated translocation assay monitors single-strand annealing following the creation of DNA double-strand breaks at multiple loci in diploid Saccharomyces cerevisiae. This mechanism may model genome rearrangements in somatic cells of higher eukaryotes following exposure to high doses of ionizing radiation.
Tags
QuantitationAnalysisFormationHO-EndonucleaseChromosomal TranslocationsSingle-Strand AnnealingSaccharomyces CerevisiaeGenetic VariationGenomic RearrangementsRepetitive ElementsEukaryotic GenomeRetrotransposon OriginLINEsSINEsExchange EventsGenome RearrangementsTranslocationsGene DosageGene ExpressionAutoimmune DiseasesCardiovascular DiseasesCancerHomologous RecombinationNon-homologous End Joining (NHEJ)Double-strand Breaks (DSBs)Mitotic CellsDNA ReplicationOxidative LesionsIonizing Radiation (IR)Exogenous DNA Damaging Agents
