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动态活细胞成像的宿主 - 病原体相互作用的研究使用的光学陷阱
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
DOI:

09:17 min

July 28, 2011

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Chapters

  • 00:05Title
  • 01:34Labeling of Pathogens with Fluorescent Dye
  • 02:52Preparation of RAW 264.7 Macrophages for Chamber Slides
  • 04:04Selection and Manipulation of a Pathogen with the Optical Trap
  • 06:18Representative Images of Pathogen and Immune Cell Interactions After Trapping-Initiated Pathogen:Cell Contact
  • 08:35Conclusion

Summary

Automatic Translation

一种方法是描述单独选择,操作和使用光学陷阱,再加上一个旋转的磁盘显微镜图像活病原体。光陷阱提供了空间和时间的控制生物体和地方他们相邻的宿主细胞。荧光显微镜捕捉动态,以最小的扰动细胞间的相互作用。

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