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Proteinler Peptid immunoaffiniti Zenginleştirme kullanarak Niceleme Kütle Spektrometresi ile birleştiğinde
JoVE Journal
Biology
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JoVE Journal Biology
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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Proteinler Peptid immunoaffiniti Zenginleştirme kullanarak Niceleme Kütle Spektrometresi ile birleştiğinde

Türkçe

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22,501 Views

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06:09 min

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July 31, 2011

DOI:

10.3791/2812-v

DOI:
10.3791/2812-v

•

06:09 min
July 31, 2011

•

3 Views
, , , , , , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, 2Department of Biochemistry and Microbiology,University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre,University of Victoria, 5Plasma Proteome Institute

Summary

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Kararlı İzotop Standartlar ve Anti-Peptid Antikorlar (SISCAPA) kendi proteinler için vekilleri olarak peptidler kantitatif ölçüm sağlamak için kararlı izotop dilüsyon kütle spektrometresi (MRM-MS) ile peptitler çiftler yakınlık zenginleştirme yakalayın. Burada kısmen otomatik bir biçimde manyetik parçacıklar kullanarak protokol açıklar.

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QuantificationProteinsPeptide Immunoaffinity EnrichmentMass SpectrometryTraditional ImmunoassaysSISCAPAStable Isotope StandardsCapture By Anti-peptide AntibodiesSRM/MRM-MSSmall MoleculesProtein ConcentrationProteotypic PeptidesTrypsin

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Cite this Article

Zhao, L., Whiteaker, J. R., Pope, M. E., Kuhn, E., Jackson, A., Anderson, N. L., Pearson, T. W., Carr, S. A., Paulovich, A. G. Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry. J. Vis. Exp. (53), e2812, doi:10.3791/2812 (2011).

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