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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
 

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Article DOI: 10.3791/2812-v • 06:09 min • July 31st, 2011
July 31st, 2011 •
*1, *1, 2, 3, 4, 5, 2, 3, 1
1Clinical Research Division, Fred Hutchinson Cancer Research Center - FHCRC, 2Department of Biochemistry and Microbiology, University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre, University of Victoria, 5Plasma Proteome Institute
* These authors contributed equally

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Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin
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