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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

JoVE Journal
Biologie

This content is Free Access.

JoVE Journal Biologie

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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06:09 min

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July 31, 2011

DOI:

DOI:

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06:09 min

July 31, 2011

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9 Views

Lei Zhao*¹, Jeffrey R. Whiteaker*¹, Matthew E. Pope, Eric Kuhn, Angela Jackson, N. Leigh Anderson, Terry W. Pearson, Steven A. Carr, Amanda G. Paulovich

¹Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, ²Department of Biochemistry and Microbiology,University of Victoria, ³Broad Institute of MIT and Harvard, ⁴Genome BC Proteomics Centre,University of Victoria, ⁵Plasma Proteome Institute

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin

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