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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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06:09 min

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July 31, 2011

DOI:

10.3791/2812-v

DOI:
10.3791/2812-v

•

06:09 min
July 31, 2011

•

3 Views
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1Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, 2Department of Biochemistry and Microbiology,University of Victoria, 3Broad Institute of MIT and Harvard, 4Genome BC Proteomics Centre,University of Victoria, 5Plasma Proteome Institute

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Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) couples affinity enrichment of peptides with stable isotope dilution mass spectrometry (MRM-MS) to provide quantitative measurement of peptides as surrogates for their respective proteins. Here we describe the protocol using magnetic particles in a partially automated format.

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QuantificationProteinsPeptide Immunoaffinity EnrichmentMass SpectrometryTraditional ImmunoassaysSISCAPAStable Isotope StandardsCapture By Anti-peptide AntibodiesSRM/MRM-MSSmall MoleculesProtein ConcentrationProteotypic PeptidesTrypsin

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Zhao, L., Whiteaker, J. R., Pope, M. E., Kuhn, E., Jackson, A., Anderson, N. L., Pearson, T. W., Carr, S. A., Paulovich, A. G. Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry. J. Vis. Exp. (53), e2812, doi:10.3791/2812 (2011).

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