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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Journal JoVE
Biologie

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Journal JoVE Biologie

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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06:09 min

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July 31, 2011

DOI:

DOI:

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06:09 min

July 31, 2011

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9 Views

Lei Zhao*¹, Jeffrey R. Whiteaker*¹, Matthew E. Pope, Eric Kuhn, Angela Jackson, N. Leigh Anderson, Terry W. Pearson, Steven A. Carr, Amanda G. Paulovich

¹Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, ²Department of Biochemistry and Microbiology,University of Victoria, ³Broad Institute of MIT and Harvard, ⁴Genome BC Proteomics Centre,University of Victoria, ⁵Plasma Proteome Institute

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin

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