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加上质谱肽免疫亲和富集的蛋白质定量

JoVE Journal
Biology

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JoVE Journal Biology

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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加上质谱肽免疫亲和富集的蛋白质定量

DOI:

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06:09 min

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July 31, 2011

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Lei Zhao*¹, Jeffrey R. Whiteaker*¹, Matthew E. Pope, Eric Kuhn, Angela Jackson, N. Leigh Anderson, Terry W. Pearson, Steven A. Carr, Amanda G. Paulovich

¹Clinical Research Division,Fred Hutchinson Cancer Research Center – FHCRC, ²Department of Biochemistry and Microbiology,University of Victoria, ³Broad Institute of MIT and Harvard, ⁴Genome BC Proteomics Centre,University of Victoria, ⁵Plasma Proteome Institute

Chapters

00:05Title
01:05Trypsin Enzymatic Digestion and Cleanup
03:17Peptide Immunoaffinity Enrichment
04:45Analysis by Multiple Reaction Monitoring – Mass Spectrometry
05:24MRM of a SISCAPA-Enriched Sample
05:44Conclusion

Summary

Automatic Translation

稳定同位素标准和捕捉反肽抗体（SISCAPA）夫妇亲和富集稳定同位素稀释质谱（MRM - MS）的肽作为代理人为各自的蛋白质肽的定量测量提供。在这里，我们描述了一个部分自动化的格式使用磁性粒子的协议。

Tags

Quantification Proteins Peptide Immunoaffinity Enrichment Mass Spectrometry Traditional Immunoassays SISCAPA Stable Isotope Standards Capture By Anti-peptide Antibodies SRM/MRM-MS Small Molecules Protein Concentration Proteotypic Peptides Trypsin

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