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Un metodo rapido per High-Resolution Imaging di fluorescenza in semi-spessa fettine di cervello
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Neuroscience
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JoVE Journal Neuroscience
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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Un metodo rapido per High-Resolution Imaging di fluorescenza in semi-spessa fettine di cervello

italiano

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16,117 Views

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04:35 min

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July 26, 2011

DOI:

10.3791/2807-v

DOI:
10.3791/2807-v

•

04:35 min
July 26, 2011

•

5 Views
, ,
1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Summary

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Qui si descrive un metodo rapido e semplice per celle di immagine fluorescente in semi-densi fettine di cervello. Fissando, affettare, e otticamente compensazione tessuto cerebrale si descrivono le modalità standard di imaging epifluorescente o confocale può essere utilizzato per visualizzare le singole cellule e reti neuronali all'interno intatto tessuto nervoso.

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Cite this Article

Selever, J., Kong, J., Arenkiel, B. R. A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices. J. Vis. Exp. (53), e2807, doi:10.3791/2807 (2011).

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