Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.
Selever, J., Kong, J., Arenkiel, B. R. A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices. J. Vis. Exp. (53), e2807, doi:10.3791/2807 (2011).