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A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

16,112 Views

04:35 min

July 26, 2011

DOI:

04:35 min
July 26, 2011

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Summary

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Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.

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