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A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

English

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16,112 Views

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04:35 min

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July 26, 2011

DOI:

10.3791/2807-v

DOI:
10.3791/2807-v

•

04:35 min
July 26, 2011

•

5 Views
, ,
1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Summary

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Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.

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Selever, J., Kong, J., Arenkiel, B. R. A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices. J. Vis. Exp. (53), e2807, doi:10.3791/2807 (2011).

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