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Een snelle aanpak van High-Resolution Imaging fluorescentie in Semi-Dik Brain Slices
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Neuroscience
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JoVE Journal Neuroscience
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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Een snelle aanpak van High-Resolution Imaging fluorescentie in Semi-Dik Brain Slices

Nederlands

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16,117 Views

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04:35 min

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July 26, 2011

DOI:

10.3791/2807-v

DOI:
10.3791/2807-v

•

04:35 min
July 26, 2011

•

5 Views
, ,
1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Summary

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Hier beschrijven we een snelle en eenvoudige methode om de afbeelding fluorescent gelabelde cellen in semi-dikke hersenen plakken. Door de vaststelling van, snijden, en optisch clearing hersenweefsel beschrijven we hoe standaard epifluorescerende of confocale beeldvorming kan worden gebruikt om individuele cellen en neuronale netwerken binnen intact zenuwweefsel te visualiseren.

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Cite this Article

Selever, J., Kong, J., Arenkiel, B. R. A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices. J. Vis. Exp. (53), e2807, doi:10.3791/2807 (2011).

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