Journal
/
神经科学
/
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

DOI:
10.3791/2807-v

•

04:35 min

•

July 26, 2011

•

, ,
1Department of Molecular & Human Genetics,Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience,Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute,Texas Children's Hospital

Chapters

  • 00:05Title
  • 01:01Agarose Embedding of Fixed Mouse Brain Tissue
  • 01:55Sectioning the Embedded Brain Tissue Using the Compresstome
  • 02:33Optical Clearing of the Brain Tissue Sections
  • 03:14Mounting the Brain Tissue Sections for Imaging
  • 03:53Epifluorescent and Confocal Microscopy of Intact Thick Brain Slices
  • 04:16Conclusion

Summary

自动翻译

自动翻译

Here we describe a rapid and simple method to image fluorescently labeled cells in semi-thick brain slices. By fixing, slicing, and optically clearing brain tissue we describe how standard epifluorescent or confocal imaging can be used to visualize individual cells and neuronal networks within intact nervous tissue.

Tags

High-resolution Fluorescence ImagingSemi-thick Brain Slices神经科学Neuroanatomical MapsMolecular GeneticsLight MicroscopyNeuronal MorphologiesMolecular And Cellular MakeupTransgenic And Gene Targeting TechnologiesFluorescent ProteinsMarine InvertebratesCell Type-specific Promoter ActivityBrain Structure And Function

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/2807?article_type=v

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain
check_url/cn/2807?article_type=v

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain
check_url/cn/2807?article_type=v

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
check_url/cn/2807?article_type=v

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
check_url/cn/2807?article_type=v

High-resolution Structural Magnetic Resonance Imaging of the Human Subcortex In Vivo and Postmortem
check_url/cn/2807?article_type=v

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin
check_url/cn/2807?article_type=v

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis
check_url/cn/2807?article_type=v

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
check_url/cn/2807?article_type=v

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
check_url/cn/2807?article_type=v

An Alternative Approach to Study Primary Events in Neurodegeneration Using Ex Vivo Rat Brain Slices

Read Article