Journal
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Derivazione di timica linfoma a cellule T Linee da Atm - / -</sup E P53 - / -</sup Mouse
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal Immunology and Infection
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
DOI:

17:59 min

April 03, 2011

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Chapters

  • 00:01Title
  • 00:05Introduction
  • 00:34Dissection of Tumor
  • 04:11Culturing Lymphoma Cells
  • 10:33Feeding the Cells
  • 11:36Passaging the Cells
  • 11:44Initial Passage
  • 15:17Maintaining Established Cell Lines
  • 16:42Freezing and Recovering the Cell Lines
  • 17:33Cell Recovery from Liquid Nitrogen

Summary

Automatic Translation

In questo video si dimostra un protocollo per stabilire le linee del mouse linfoma delle cellule del timo. Seguendo questo protocollo, abbiamo stabilito con successo diverse linee di cellule T da Atm-/ - e p53-/ - i topi con linfoma del timo.

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