In this video we demonstrate a protocol to establish mouse thymic lymphoma cell lines. By following this protocol, we have successfully established several T-cell lines from Atm-/- and p53-/- mice with thymic lymphoma.
Jinadasa, R., Balmus, G., Gerwitz, L., Roden, J., Weiss, R., Duhamel, G. Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice. J. Vis. Exp. (50), e2598, doi:10.3791/2598 (2011).