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Preparation and Applications of Organotypic Thymic Slice Cultures
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation and Applications of Organotypic Thymic Slice Cultures

Preparation and Applications of Organotypic Thymic Slice Cultures

English

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7,806 Views

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10:10 min

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August 06, 2016

DOI:

10.3791/54355-v

DOI:
10.3791/54355-v

•

10:10 min
August 06, 2016

•

2 Views
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1Centre de Recherche,Hôpital Maisonneuve-Rosemont, 2Department of Microbiology, Infectiology and Immunology,Université de Montréal, 3Department of Medicine,Université de Montréal

Summary

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We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.

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Cite this Article

Sood, A., Dong, M., Melichar, H. J. Preparation and Applications of Organotypic Thymic Slice Cultures. J. Vis. Exp. (114), e54355, doi:10.3791/54355 (2016).

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