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Immunology and Infection
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胸腺淋巴瘤的T细胞系的推导 ATM - / -</sup P53 - / -</sup鼠标
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Immunology and Infection
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JoVE Journal Immunology and Infection
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
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胸腺淋巴瘤的T细胞系的推导 ATM - / -</sup P53 - / -</sup鼠标

DOI:
10.3791/2598-v

•

17:59 min

•

April 03, 2011

•

, , , , ,
1Department of Biomedical Sciences,Cornell University

Chapters

  • 00:01Title
  • 00:05Introduction
  • 00:34Dissection of Tumor
  • 04:11Culturing Lymphoma Cells
  • 10:33Feeding the Cells
  • 11:36Passaging the Cells
  • 11:44Initial Passage
  • 15:17Maintaining Established Cell Lines
  • 16:42Freezing and Recovering the Cell Lines
  • 17:33Cell Recovery from Liquid Nitrogen

Summary

Automatic Translation

Automatic Translation

在这个视频中，我们展示了一个协议来建立小鼠胸腺淋巴瘤细胞株。通过这个协议，我们已经成功地建立了几个从ATM / T细胞线 - 和p53 - / - 小鼠胸腺淋巴瘤。

Tags

Thymic LymphomaT-cell LinesAtm-/- MiceP53-/- MiceGenetically Modified MiceLaboratory AnimalsResearch ToolMurine T-cell LinesInterleukinsProtocolTumorsVisible TumorsHunched PostureLabored BreathingPoor GroomingWastingSusceptible StrainInbred StrainsCD3 ExpressionCD4 ExpressionCD8 ExpressionPassaged

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