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Chapters
- 00:05Title
- 00:42Fabrication of Glass Needles and Wire Needles
- 02:15Calibration of the Intermediate and Working Glass Needles
- 03:42Coating Needles with Attachment Factors
- 04:13Equipment Set Up and Needles Alignment
- 06:15Cell Attachment
- 07:19Axon Extension and Data Analysis
- 08:13Representative Results for Controlling Axonal Development
- 09:43Conclusion
Toepassing en directe metingen van de krachten op neuronen in de 2-1000 microdyne serie worden bereikt met een hoge precisie met geijkte glas naalden. Deze methodiek kan gebruikt worden om de controle en meten van verschillende aspecten van axonale ontwikkeling, waaronder axonale initiatie, axonale spanning, snelheid van axonale rek, en kracht vectoren.
Tags
Mechanical ManipulationNeuronsControlAxonal DevelopmentGlass Micro-fibersForcesHookean BendingCalibrationEquipment RequirementsProceduresFabricatingTreatingCellsForce RegimesCell TypesFlexibilityMethodologyInvestigationVisualizationDirect InterventionCellular EventsStimulationRegulationAxonal GrowthRetractionDetachment
