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DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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JoVE Journal Neuroscience
DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

DOI:
10.3791/3667-v

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09:14 min

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December 13, 2011

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1Developmental Neurobiology,Max Delbrück Center for Molecular Medicine

Chapters

  • 00:05Title
  • 01:56Dissection Procedure
  • 04:32DiI Labeling of DRG Neurons
  • 06:29Mounting and Microscopic Analysis
  • 07:27Results: Visualizing and Quantification of Single Axonal Trajectories of Embryonic Mouse DRG Neurons
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.

Tags

DiI-labelingDRG NeuronsAxonal BranchingWhole Mount PreparationMouse Embryonic Spinal CordDiffusive StainingLipophilic Tracer11′-dioctadecyl-333′3′-tetramethylindocarbocyanine Perchlorate (DiI)Axonal PathwaysWild-typeMutated Mouse LinesFunctional RoleCandidate ProteinsControl Of Axonal BranchingWiring Of The Nervous SystemParallel Processing Of InformationIntermediate Target RegionsTerminal ArborizationAxonal GrowthGrowth ConeSplitting BranchingDelayed BranchingInterstitial BranchingCentral Projections Of NeuronsDorsal Root Entry Zone (DREZ)Stereotyped Pattern

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