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DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord
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December 13, 2011
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Summary
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The stereotyped projections of sensory afferents into the rodent spinal cord offer an easily accessible experimental system to study axonal branching through the tracing of single axons.
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DiI-labelingDRG NeuronsAxonal BranchingWhole Mount PreparationMouse Embryonic Spinal CordDiffusive StainingLipophilic Tracer11′-dioctadecyl-333′3′-tetramethylindocarbocyanine Perchlorate (DiI)Axonal PathwaysWild-typeMutated Mouse LinesFunctional RoleCandidate ProteinsControl Of Axonal BranchingWiring Of The Nervous SystemParallel Processing Of InformationIntermediate Target RegionsTerminal ArborizationAxonal GrowthGrowth ConeSplitting BranchingDelayed BranchingInterstitial BranchingCentral Projections Of NeuronsDorsal Root Entry Zone (DREZ)Stereotyped Pattern
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Schmidt, H., Rathjen, F. G. DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord. J. Vis. Exp. (58), e3667, doi:10.3791/3667 (2011).
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