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Chapters
- 00:05Title
- 00:42Fabrication of Glass Needles and Wire Needles
- 02:15Calibration of the Intermediate and Working Glass Needles
- 03:42Coating Needles with Attachment Factors
- 04:13Equipment Set Up and Needles Alignment
- 06:15Cell Attachment
- 07:19Axon Extension and Data Analysis
- 08:13Representative Results for Controlling Axonal Development
- 09:43Conclusion
Application and direct measurements of forces on neurons in the 2-1000 microdyne range are achieved with high precision using calibrated glass needles. This methodology can be used to control and measure several aspects of axonal development, including axonal initiation, axonal tension, velocity of axonal elongation, and force vectors.
Tags
Mechanical ManipulationNeuronsControlAxonal DevelopmentGlass Micro-fibersForcesHookean BendingCalibrationEquipment RequirementsProceduresFabricatingTreatingCellsForce RegimesCell TypesFlexibilityMethodologyInvestigationVisualizationDirect InterventionCellular EventsStimulationRegulationAxonal GrowthRetractionDetachment
