ジャーナル
/
免疫学と感染
/
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

DOI:
10.3791/2355-v

•

07:25 min

•

December 26, 2010

•

, , , ,
1Department of Medical Microbiology and Immunology,University of California, Davis, 2Département Génétique et Développement, Institut Cochin,Université Paris Descartes

章

  • 00:05標題
  • 01:19Transfection and Mutagenesis of Target Genes in Mosquito Cells
  • 04:14Post-transfection Cell Maintenance and Analysis
  • 05:46Representative Results for Transfection and Mutagenesis of Target Genes in Mosquito Cells by LNA-ONs
  • 07:00Conclusion

概要

自動翻訳

自動翻訳

Oligonucleotides can be used to site specifically substitute a single nucleotide of transfected target genes in both Anopheles gambiae and Anopheles stephensi cells.

タグ

TransfectionMutagenesisTarget GenesMosquito CellsLocked Nucleic Acid-modified OligonucleotidesPlasmodium ParasitesMalariaGenetically Modified MosquitoesCell Signaling PathwaysTargeted MutagenesisGene TargetsHomologous RecombinationDNA MoleculeStably Transformed Cell LinesLocked Nucleic Acid-modified Oligonucleotides (LNA-ONs)Artificial Single Nucleotide Substitutions

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Identifying Dysregulated Genes Induced by Kaposi’s Sarcoma-associated Herpesvirus (KSHV)

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

Optimized Protocol for Efficient Transfection of Dendritic Cells without Cell Maturation

Introducing Shear Stress in the Study of Bacterial Adhesion

Following Cell-fate in E. coli After Infection by Phage Lambda

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Locked Nucleic Acid Flow Cytometry-fluorescence in situ Hybridization (LNA flow-FISH): a Method for Bacterial Small RNA Detection

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Highly Efficient Transfection of Human THP-1 Macrophages by Nucleofection

Read Article