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Transfección y mutagénesis de los genes diana en células de mosquito por nucleico cerrado modificado ácido oligonucleótidos
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Immunology and Infection
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JoVE Journal Immunology and Infection
Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
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Transfección y mutagénesis de los genes diana en células de mosquito por nucleico cerrado modificado ácido oligonucleótidos

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10,287 Views

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07:25 min

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December 26, 2010

DOI:

10.3791/2355-v

DOI:
10.3791/2355-v

•

07:25 min
December 26, 2010

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2 Views
, , , ,
1Department of Medical Microbiology and Immunology,University of California, Davis, 2Département Génétique et Développement, Institut Cochin,Université Paris Descartes

Summary

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Oligonucleótidos se puede utilizar para sustituir a un sitio concreto de un solo nucleótido de los genes diana transfectadas en ambos<em> Anopheles gambiae</em> Y<em> Anopheles stephensi</em> Células.

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Pakpour, N., Cheung, K. W., Souvannaseng, L., Concordet, J., Luckhart, S. Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides. J. Vis. Exp. (46), e2355, doi:10.3791/2355 (2010).

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