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弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
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Immunology and Infection
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JoVE Journal Immunology and Infection
Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
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弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件

DOI:
10.3791/2091-v

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09:56 min

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August 12, 2010

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, ,
1Department of Medical Microbiology and Immunology,University of Wisconsin

Chapters

  • 00:04Title
  • 00:44Introduction
  • 01:10Preparation of HFF-coated Coverslips
  • 02:37Infecting Cells
  • 03:57Detection of Bradyzoites
  • 05:48Inducing Cyst Wall Formation
  • 09:08Representative Results
  • 09:30Conclusion

Summary

Automatic Translation

Automatic Translation

弓形虫囊肿的形式，在应对环境压力，可在组织培养模式模仿的转换。本视频演示了技术审查囊肿壁的形成，激活骨髓来源的巨噬细胞或改变成纤维细胞生长介质的pH值。

Tags

Toxoplasma GondiiCyst Wall FormationBone Marrow-derived MacrophagesBradyzoite ConditionsObligate Intracellular ParasiteTachyzoiteEncysted FormSignaling ProcessChronic Stage Of InfectionCO2 StarvationHigh PH MediumInterferon Gamma (IFNγ)Dolichos Biflorus Agglutinin (DBA)Fluorescently Labeled DBAHuman Foreskin Fibroblasts (HFFs)Murine Bone Marrow-derived Macrophages (BMMs)Lipopolysaccharide (LPS)High PH Growth Medium

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