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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

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16,784 Views

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09:52 min

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July 12, 2013

DOI:

10.3791/50598-v

DOI:
10.3791/50598-v

•

09:52 min
July 12, 2013

•

6 Views
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1Department of Microbiology and Immunology,The Geisel School of Medicine at Dartmouth

Summary

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Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.

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Genetic ManipulationΔKU80 StrainsToxoplasma GondiiFunctional Genomic AnalysisHomologous RecombinationGene DeletionGene ReplacementGene TaggingHXGPRT Selectable Marker

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Rommereim, L. M., Hortua Triana, M. A., Falla, A., Sanders, K. L., Guevara, R. B., Bzik, D. J., Fox, B. A. Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii. J. Vis. Exp. (77), e50598, doi:10.3791/50598 (2013).

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