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BRET Emisyon Testi için pCMV5-NanoLuc-CRAF ve pCMV5-14-3-3ζ-Halo ile Transfekte Edilmiş 293FT Hücrelerinin Hazırlanması

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Preparing 293FT Cells Transfected with pCMV5-NanoLuc-CRAF and pCMV5-14-3-3ζ-Halo for BRET Emission Assay

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BRET Emisyon Testi için pCMV5-NanoLuc-CRAF ve pCMV5-14-3-3ζ-Halo ile Transfekte Edilmiş 293FT Hücrelerinin Hazırlanması

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03:39 min

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March 01, 2024

DOI:

10.3791/201480-v

DOI:

10.3791/201480-v

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03:39 min

March 01, 2024

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1 Views

Russell Spencer-Smith^1,2,3

¹Department of Cell and Molecular Pharmacology and Experimental Therapeutics,Medical University of South Carolina, ²Hollings Cancer Center,Medical University of South Carolina, ³Laboratory of Cell and Developmental Signaling, Center for Cancer Research,National Cancer Institute-Frederick

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