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补消耗的特定细胞群的枯竭
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Depletion of Specific Cell Populations by Complement Depletion
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补消耗的特定细胞群的枯竭

DOI:
10.3791/1487-v

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06:17 min

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February 05, 2010

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1BloodCenter of Wisconsin,Blood Research Institute

Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:18Preparation of Rabbit Complement and Antibody Selection
  • 02:22Complement Depletion
  • 05:06Results of the Complement Depletion
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

为了有效地研究其净化往往需要的人群的免疫细胞的功能。补枯竭是一个快速和廉价的技术，高纯度的免疫细胞群的隔离。

Tags

DepletionSpecific Cell PopulationsComplement DepletionImmune Cell PurificationReal-time PCRMicroarray AnalysisFluorescent Activated Cell Sorting (FACS)Magnetic Bead SeparationComplement SystemPlasma ProteinsProteolytic CascadeMembrane-attack ComplexCell DeathClassical PathwayIgM AntibodiesIgG AntibodiesMonoclonal Antibodies (mAb)Antigen-specific MannerRabbit ComplementIncubationWashing

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