/
/
Measuring the 50% Haemolytic Complement (CH50) Activity of Serum
A subscription to JoVE is required to view this content. Sign in or start your free trial.
36,887 Views
•
08:26 min
•
March 29, 2010
DOI:
Summary
Automatically generated
The classical pathway is activated by antibody and culminates in target cell lysis. The CH50 assay provides a measure of the complement activity of a serum sample. This video demonstrates the steps involved in determining the CH50 of a serum sample, the calculations and interpreting of results.
Explore More Videos
50% Haemolytic ComplementCH50 ActivityComplement SystemTarget Cell LysisPhagocytosisOpsonisationClassical Complement PathwayScreening AssaySerum Complement ComponentsSheep Red Blood CellsRabbit Anti-sheep Red Blood Cell AntibodyHaemolysisComplement Component Absence Or ReductionHaemoglobin AbsorbanceComplement Activity Analysis
Read Article
Cite this Article
Costabile, M. Measuring the 50% Haemolytic Complement (CH50) Activity of Serum. J. Vis. Exp. (37), e1923, doi:10.3791/1923 (2010).
Copy