/
/
Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,333 Views
•
08:48 min
•
October 25, 2016
DOI:
Summary
Automatically generated
Herein we describe a novel method to generate antigen-specific T cell receptors (TCRs) by pairing the TCRα or TCRβ of an existing TCR, possessing the antigen-specificity of interest, with complementary hemichain of the peripheral T cell receptor repertoire. The de novo generated TCRs retain antigen-specificity with varying affinity.
Read Article
Cite this Article
Guo, T., Ochi, T., Nakatsugawa, M., Kagoya, Y., Anczurowski, M., Wang, C., Rahman, M. A., Saso, K., Butler, M. O., Hirano, N. Generating De Novo Antigen-specific Human T Cell Receptors by Retroviral Transduction of Centric Hemichain. J. Vis. Exp. (116), e54697, doi:10.3791/54697 (2016).
Copy