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Una lectina Método HPLC para enriquecer péptidos selectivamente glicosilada de muestras biológicas complejas
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Biology
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JoVE Journal Biology
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
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Una lectina Método HPLC para enriquecer péptidos selectivamente glicosilada de muestras biológicas complejas

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15,806 Views

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20:23 min

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October 01, 2009

DOI:

10.3791/1398-v

DOI:
10.3791/1398-v

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20:23 min
October 01, 2009

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6 Views
, , , , , , , , , , , ,
1Obstetrics, Gynecology and Reproductive Sciences,University of California, San Francisco – UCSF, 2Buck Institute for Age Research, 3Department of Chemistry,Purdue University

Summary

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Lectina conjugada con perlas de Poros fueron empleados para HPLC. Normas glicopéptido sirvieron como controles positivos y negativos. MARS-14 empobrecido, tripsina digerido plasma humano y se sometió a cromatografía de flujo continuo (FT) y las fracciones recogidas obligado para el análisis de ESI-LC-MS/MS. Glicopéptidos fueron enriquecidos en la fracción unida en comparación con el FT.

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Cite this Article

Johansen, E., Schilling, B., Lerch, M., Niles, R. K., Liu, H., Li, B., Allen, S., Hall, S. C., Witkowska, H. E., Regnier, F. E., Gibson, B. W., Fisher, S. J., Drake, P. M. A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples. J. Vis. Exp. (32), e1398, doi:10.3791/1398 (2009).

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