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October 01, 2009
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Contas lectina-conjugada POROS foram empregadas para HPLC. Padrões glicopeptídeo serviram como controle positivo e negativo. MARS-14 empobrecido, tripsina-digerida plasma humano foi cromatografada e flow-through (FT) e frações ligado coletadas para análises ESI-LC-MS/MS. Glicopeptídeos foram enriquecidos na fração ligada quando comparado com FT.
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Johansen, E., Schilling, B., Lerch, M., Niles, R. K., Liu, H., Li, B., Allen, S., Hall, S. C., Witkowska, H. E., Regnier, F. E., Gibson, B. W., Fisher, S. J., Drake, P. M. A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples. J. Vis. Exp. (32), e1398, doi:10.3791/1398 (2009).
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