This content is Free Access.
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
15,800 Views
•
20:23 min
•
October 01, 2009
DOI:
Özet
Automatically generated
Lectin-conjugated POROS beads were employed for HPLC. Glycopeptide standards served as positive and negative controls. MARS-14 depleted, trypsin-digested human plasma was chromatographed and flow-through (FT) and bound fractions collected for ESI-LC-MS/MS analyses. Glycopeptides were enriched in the bound fraction as compared to FT.
Explore More Videos
Lectin HPLC MethodSelectively-glycosylated PeptidesComplex Biological SamplesGlycansPost-translational ModificationsTumor CellsCancer-associated Glycan VariantsBiomarker DevelopmentLectinsSambucus Nigra (SNA)Aleuria Aurantia (AAL)Affinity MatricesPOROS BeadsPEEK ColumnsHigh Pressure Liquid Chromatography (HPLC)Plasma DepletionMultiple Affinity Removal System (MARS-14)Trypsin-digestionFlow-through FractionBound FractionPNGaseFN-linked GlycansLC-MS/MS AnalysisQStar EliteMascot SoftwarePositive ControlsNegative Controls
Read Article
Cite this Article
Johansen, E., Schilling, B., Lerch, M., Niles, R. K., Liu, H., Li, B., Allen, S., Hall, S. C., Witkowska, H. E., Regnier, F. E., Gibson, B. W., Fisher, S. J., Drake, P. M. A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples. J. Vis. Exp. (32), e1398, doi:10.3791/1398 (2009).
Copy