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A Lectin HPLC-Methode zur selektiven glykosylierte Peptide aus komplexen biologischen Proben Enrich
JoVE Journal
Biology
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JoVE Journal Biology
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
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A Lectin HPLC-Methode zur selektiven glykosylierte Peptide aus komplexen biologischen Proben Enrich

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15,794 Views

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20:23 min

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October 01, 2009

DOI:

10.3791/1398-v

DOI:
10.3791/1398-v

•

20:23 min
October 01, 2009

•

6 Views
, , , , , , , , , , , ,
1Obstetrics, Gynecology and Reproductive Sciences,University of California, San Francisco – UCSF, 2Buck Institute for Age Research, 3Department of Chemistry,Purdue University

Summary

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Lectin-konjugierten POROS Perlen wurden für die HPLC eingesetzt. Glycopeptid-Normen diente als positive und negative Kontrollen. MARS-14 aufgebraucht wurde trypsinverdauten Humanplasma chromatographiert und Flow-Through-(FT) und gebundenen Fraktionen für ESI-LC-MS/MS Analysen gesammelt. Glykopeptide wurden in der gebundenen Fraktion angereichert, um FT verglichen.

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Johansen, E., Schilling, B., Lerch, M., Niles, R. K., Liu, H., Li, B., Allen, S., Hall, S. C., Witkowska, H. E., Regnier, F. E., Gibson, B. W., Fisher, S. J., Drake, P. M. A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples. J. Vis. Exp. (32), e1398, doi:10.3791/1398 (2009).

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