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BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
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JoVE Journal Nörobilim
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

DOI:
10.3791/65063-v

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05:17 min

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May 26, 2023

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, , , ,
1Centre for Addiction and Mental Health (CAMH),Campbell Family Mental Health Research Institute, 2Department of Pharmacology and Toxicology,University of Toronto, 3Psikiyatri Bölümü,University of Toronto

Bölümler

  • 00:04Introduction
  • 00:41Brain Tissue Dissection and Crosslinking Reaction
  • 03:36Results: Effect of BS3 Crosslinking and UCMS on the α5-GABAAR Expression Level
  • 04:36Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress conditions.

Etiketler

BS3 Chemical Crosslinking AssayGABAA ReceptorCell Surface PresentationRodent BrainPsychotropic AgentsPsychogenic StressBrain Region-specific EvaluationReceptor DynamicsPrefrontal CortexHippocampusCrosslinking Reaction

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