Journal
/
Neurowissenschaften
/
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

DOI:
10.3791/65063-v

•

05:17 min

•

May 26, 2023

•

, , , ,
1Centre for Addiction and Mental Health (CAMH),Campbell Family Mental Health Research Institute, 2Department of Pharmacology and Toxicology,University of Toronto, 3Department of Psychiatry,University of Toronto

Kapitel

  • 00:04Introduction
  • 00:41Brain Tissue Dissection and Crosslinking Reaction
  • 03:36Results: Effect of BS3 Crosslinking and UCMS on the α5-GABAAR Expression Level
  • 04:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress conditions.

Tags

BS3 Chemical Crosslinking AssayGABAA ReceptorCell Surface PresentationRodent BrainPsychotropic AgentsPsychogenic StressBrain Region-specific EvaluationReceptor DynamicsPrefrontal CortexHippocampusCrosslinking Reaction

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Simultaneous fMRI and Electrophysiology in the Rodent Brain

Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Personalized Needles for Microinjections in the Rodent Brain

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Read Article