Journal
/
Neurociencias
/
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

DOI:
10.3791/65063-v

•

05:17 min

•

May 26, 2023

•

, , , ,
1Centre for Addiction and Mental Health (CAMH),Campbell Family Mental Health Research Institute, 2Department of Pharmacology and Toxicology,University of Toronto, 3Department of Psychiatry,University of Toronto

Capítulos

  • 00:04Introduction
  • 00:41Brain Tissue Dissection and Crosslinking Reaction
  • 03:36Results: Effect of BS3 Crosslinking and UCMS on the α5-GABAAR Expression Level
  • 04:36Conclusion

Summary

Traducción Automática

Traducción Automática

The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress conditions.

Tags

BS3 Chemical Crosslinking AssayGABAA ReceptorCell Surface PresentationRodent BrainPsychotropic AgentsPsychogenic StressBrain Region-specific EvaluationReceptor DynamicsPrefrontal CortexHippocampusCrosslinking Reaction

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Simultaneous fMRI and Electrophysiology in the Rodent Brain

Brain Imaging Investigation of the Impairing Effect of Emotion on Cognition

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

Personalized Needles for Microinjections in the Rodent Brain

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis

Read Article