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BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
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神经科学
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JoVE 杂志 神经科学
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain

DOI:
10.3791/65063-v

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05:17 min

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May 26, 2023

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, , , ,
1Centre for Addiction and Mental Health (CAMH),Campbell Family Mental Health Research Institute, 2Department of Pharmacology and Toxicology,University of Toronto, 3Department of Psychiatry,University of Toronto

Chapters

  • 00:04Introduction
  • 00:41Brain Tissue Dissection and Crosslinking Reaction
  • 03:36Results: Effect of BS3 Crosslinking and UCMS on the α5-GABAAR Expression Level
  • 04:36Conclusion

Summary

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The BS3 chemical crosslinking assay reveals reduced cell surface GABAA receptor expression in mouse brains under chronic psychosocial stress conditions.

Tags

Keywords: BS3 Chemical Crosslinking AssayGABAA ReceptorCell Surface PresentationRodent BrainPsychotropic AgentsPsychogenic StressBrain Region-specific EvaluationReceptor DynamicsPrefrontal CortexHippocampusCrosslinking Reaction

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