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使用二分法减少牛卵母细胞中的线粒体DNA
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JoVE Journal Gelişim Biyolojisi
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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使用二分法减少牛卵母细胞中的线粒体DNA

1,879 Views

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06:15 min

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July 06, 2022

DOI:

10.3791/64060-v

DOI:
10.3791/64060-v

•

06:15 min
July 06, 2022

•

8 Views
, , , , , ,
1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, 3Genus PLC

Özet

Automatically generated

在这里，我们提出了一种方案，以显着减少牛卵母细胞中的线粒体DNA拷贝数（P <0.0001）。该方法利用离心和二分切来大大减少卵母细胞线粒体，并且可以增加在重建的种间体细胞核移植胚胎中发育的机会。

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Cite this Article

Adams, L., Liu, Y., Durrant, B., Ruggeri, E., Young, C., Krisher, R., Polejaeva, I. Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte. J. Vis. Exp. (185), e64060, doi:10.3791/64060 (2022).

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