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通过代谢标记和薄层色谱分析 糖浆 中中性脂质合成
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JoVE Journal Biyokimya
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
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通过代谢标记和薄层色谱分析 糖浆 中中性脂质合成

DOI:
10.3791/62201-v

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08:30 min

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February 02, 2021

•

,
1Department of Cell Biology,Teksas Üniversitesi Southwestern Tıp Merkezi

Bölümler

  • 00:04Introduction
  • 00:30Growth and Labelling of Yeast Cells
  • 02:55Isolation of Total Lipids from Yeast
  • 04:42Thin Layer Chromatography and Quantification of Results
  • 07:09Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
  • 08:03Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里，为酵母的代谢标记提供 14个C-醋酸，这与薄层色谱相结合，用于中性脂质的分离。

Etiketler

Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

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