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Klamidya trachomatis Floxed Kaset Allelic Exchange Mutagene tarafından Markerless Gen Deletion
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Klamidya trachomatis Floxed Kaset Allelic Exchange Mutagene tarafından Markerless Gen Deletion
Bölümler
- 00:04Introduction
- 00:27Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
- 02:20Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
- 05:54Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
- 07:38Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
- 08:42Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects
- 09:47Conclusion
