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Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

6,457 Views

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10:35 min

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January 30, 2020

DOI:

10.3791/60848-v

DOI:
10.3791/60848-v

•

10:35 min
January 30, 2020

•

8 Views
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1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Summary

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Described here is a method for targeted, markerless gene deletion in Chlamydia trachomatis using floxed cassette allelic exchange mutagenesis, FLAEM.

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Markerless Gene DeletionFloxed CassetteAllelic Exchange MutagenesisChlamydia TrachomatisHomologous RecombinationPCRDNA AssemblyE. Coli TransformationMcCoy CellsChlamydia Infection

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Cite this Article

Keb, G., Fields, K. A. Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis. J. Vis. Exp. (155), e60848, doi:10.3791/60848 (2020).

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