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Markerless Gene Deletion von Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Markerless Gene Deletion von Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
Bölümler
- 00:04Introduction
- 00:27Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
- 02:20Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
- 05:54Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
- 07:38Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
- 08:42Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects
- 09:47Conclusion
