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Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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Immunologie et infection
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Journal JoVE Immunologie et infection
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

6,457 Views

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10:35 min

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January 30, 2020

DOI:

10.3791/60848-v

DOI:
10.3791/60848-v

•

10:35 min
January 30, 2020

•

8 Views
,
1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Summary

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Described here is a method for targeted, markerless gene deletion in Chlamydia trachomatis using floxed cassette allelic exchange mutagenesis, FLAEM.

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Markerless Gene DeletionFloxed CassetteAllelic Exchange MutagenesisChlamydia TrachomatisHomologous RecombinationPCRDNA AssemblyE. Coli TransformationMcCoy CellsChlamydia Infection

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Cite this Article

Keb, G., Fields, K. A. Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis. J. Vis. Exp. (155), e60848, doi:10.3791/60848 (2020).

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