Rivista
/
Immunologia e infezioni
/
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

6,457 Views

•

10:35 min

•

January 30, 2020

DOI:

10.3791/60848-v

DOI:
10.3791/60848-v

•

10:35 min
January 30, 2020

•

8 Views
,
1Department of Microbiology, Immunology, and Molecular Genetics,University of Kentucky

Summary

Automatically generated

Described here is a method for targeted, markerless gene deletion in Chlamydia trachomatis using floxed cassette allelic exchange mutagenesis, FLAEM.

Explore More Videos

Markerless Gene DeletionFloxed CassetteAllelic Exchange MutagenesisChlamydia TrachomatisHomologous RecombinationPCRDNA AssemblyE. Coli TransformationMcCoy CellsChlamydia Infection

Read Article

Cite this Article

Keb, G., Fields, K. A. Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis. J. Vis. Exp. (155), e60848, doi:10.3791/60848 (2020).

Download .ris file

Copy

Share Video

.

Copy