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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应
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İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
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巨噬细胞 ros 产生的流式细胞仪测量对 fcγr 交联的响应

DOI:
10.3791/59167-v

•

13:20 min

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March 07, 2019

•

,
1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences,University of Central Florida

Bölümler

  • 00:04Başlık
  • 01:22Reagent and Cell Preparation for ROS Measurement
  • 05:02Assay Preparation
  • 05:53Performing the Assay
  • 11:28Results: Flow Cytometric Measurement of ROS Production in Macrophages in Response to FcγR Cross-linking
  • 13:00Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

这项研究表明, 使用流式细胞仪检测活性氧 (ros) 的产生, 由于激活的 fcγr。该方法可用于评估吞噬细胞对免疫复合物、光化微生物或直接 fcγr 交联的抗菌和氧化还原信号功能的变化。

Etiketler

Flow CytometryROS ProductionMacrophagesFc-gamma Receptor CrosslinkingOxidative StressSuperoxideChronic Granulomatous DiseaseAutoimmune DiseasesNeurodegenerative Diseases

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