Assessing Lysosomal Alkalinization in the Intestine of Live <em>Caenorhabditis elegans</em>

Kunal Baxi; Carlos E. de Carvalho

doi:10.3791/57414

Dergi

/

Gelişim Biyolojisi

/

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

JoVE Journal
Gelişim Biyolojisi

Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.

JoVE Journal Gelişim Biyolojisi

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

DOI:

10.3791/57414-v

•

06:18 min

•

April 13, 2018

•

Kunal Baxi, Carlos E. de Carvalho

¹Biyoloji Bölümü,University of Saskatchewan

Bölümler

00:04Başlık
01:01Staining and Imaging of Intestinal Lysosomes
04:24Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
05:38Conclusion

Özet

Otomatik Çeviri

A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

Etiketler

Lysosomal Alkalinization Caenorhabditis Elegans Autophagy Lysosomal Function Protein Degradation CDCFDA Staining OP50 Bacteria NGM Plates M9 Buffer Agarose Pad Sodium Azide

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans

Spatial and Temporal Analysis of Active ERK in the C. elegans Germline

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Live Imaging of Mouse Secondary Palate Fusion

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging