/
/
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
5,848 Views
•
06:18 min
•
April 13, 2018
DOI:
Summary
Automatically generated
A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)
Read Article
Cite this Article
Baxi, K., de Carvalho, C. E. Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans. J. Vis. Exp. (134), e57414, doi:10.3791/57414 (2018).
Copy